As I often get an inquiry how we do the NGS analysis, especially RNA-seq, so here is what I write it down as my memorandum.
For the outline of the NGS analysis, this web site contains a good summary and video:
https://www.abmgood.com/marketing/knowledge_base/next_generation_sequencing_data_analysis.php
Above doesn't talk much about RNA-seq, so I'm including some presentation
http://media.soph.uab.edu/ssg/ssg-shortcourse-20151214-17/ssg-shortcourse-cui-20151216/html5.html
https://www.youtube.com/watch?v=7_yY-PuVN3U
https://www.youtube.com/watch?v=faFNJ1wXx2w
The above 2 youtubes are close to what we are actually doing here.
The image is a screenshot of (1) initial bcl2fastq conversion to make fastq files out from HiSeq run directory.
